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Table 4 Mode of inhibition of rat liver FAAH-1 oleamide hydrolysis by indomethacin and meclofenamate

From: Assessment of NSAIDs as potential inhibitors of the fatty acid amide hydrolase I (FAAH-1) using three different primary fatty acid amide substrates in vitro

FAAH-1 Kinetics Km (μM) Vmax (nmol/min/mg protein) Substrate
Control 18.4 ± 3.5 4.6 ± 0.5 Oleamide
+ 200 μM indomethacin 24.4 ± 3.3 3.8 ± 0.4 Oleamide
+ 100 μM meclofenamate 22.7 ± 1.4 2.9 ± 0.1 Oleamide
Control 19.8 ± 2.0 8.4 ± 1.2 Arachidonamide
+ 200 μM indomethacin 21.6 ± 2.8 7.2 ± 0.9 Arachidonamide
+ 100 μM meclofenamate 23.4 ± 3.7 6.7 ± 1.0 Arachidonamide
  1. Data are mean ± SEM of triplicate assessments conducted on five transient transfects (n = 5)