Assessment of NSAIDs as potential inhibitors of the fatty acid amide hydrolase I (FAAH-1) using three different primary fatty acid amide substrates in vitro

Table 4 Mode of inhibition of rat liver FAAH-1 oleamide hydrolysis by indomethacin and meclofenamate

FAAH-1 Kinetics	K_m (μM)	V_max (nmol/min/mg protein)	Substrate
Control	18.4 ± 3.5	4.6 ± 0.5	Oleamide
+ 200 μM indomethacin	24.4 ± 3.3	3.8 ± 0.4	Oleamide
+ 100 μM meclofenamate	22.7 ± 1.4	2.9 ± 0.1	Oleamide
Control	19.8 ± 2.0	8.4 ± 1.2	Arachidonamide
+ 200 μM indomethacin	21.6 ± 2.8	7.2 ± 0.9	Arachidonamide
+ 100 μM meclofenamate	23.4 ± 3.7	6.7 ± 1.0	Arachidonamide

Data are mean ± SEM of triplicate assessments conducted on five transient transfects (n = 5)

ISSN: 2050-6511