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Table 2 Lethality and signs of toxicity in mice administered with nicotine tested by iUDP

From: Improved up-and-down procedure for acute toxicity measurement with reliable LD50 verified by typical toxic alkaloids and modified Karber method

Seq. Dosage (mg/kg) Δm (g) Short-term outcome Symptoms Pathology
1 12.6 1.1 O Convulsive, weakness, recovered after 2 h No visible alterations were found in organs and tissues
2 20 1.5 O Violently convulsive, recovered after 2 h Spleen was enlarged and congested
3 32 1.4 O Violently convulsive, weakness, recovered after 6 h Lung was enlarged and congested
4 50 0.9 X Violently convulsive, dead after 5 min Heart and lung were enlarged
5 32 1.1 O Violently convulsive, weakness, recovered after 6 h Heart and lung were markedly enlarged
6 50 1.7 X Violently convulsive, dead after 10 min Heart, liver and lung were enlarged
7 32 1.4 X Violently convulsive, dead after 5 min Heart, liver and lung were enlarged
Stop criteria met: 3 reversals in 5 tests
  1. Note: The sequence of outcomes: O for alive and X for dead