Skip to main content
Fig. 4 | BMC Pharmacology and Toxicology

Fig. 4

From: Novel dihydroartemisinin derivative Mito-DHA5 induces apoptosis associated with mitochondrial pathway in bladder cancer cells

Fig. 4

Effects of Mito-DHA5 on ROS production. A T24 cells were treated with Mito-DHA5 (0, 3, 10 µM) in the presence or absence of pretreated with 5 mM NAC for 24 h, then DCFH-DA (10 µM) was loaded and cells were analyzed by fluorescence microscopy. Scale bar was 100 μm. B Average fluorescence intensity of different treatment groups. C Effects of NAC on Mito-DHA5-induced cytotoxicity in T24 cells assessed by MTT. Cells were treated with Mito-DHA5 (3, 10, 30, 100 µM) for 72 h in the presence or absence of pretreated with 5 mM NAC. D T24 Cells were treated with Mito-DHA5 (10 µM) in the presence or absence of pretreated with 5 mM NAC for 48 h, stained with Hochest 33,258, and viewed by fluorescence microscopy. Scale bar 100 was µm. Data are presented as mean ±SD. *P<0.5, **P<0.01, ***P<0.001, #P<0.5, ##P<0.01, ###P<0.001

Back to article page