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Table 6 Ames test results of Py-mulin using S. typhimurium strains TA97, TA98, TA100, TA102 and TA1535

From: Acute, subacute oral toxicity and Ames test of Py-mulin: an antibacterial drug candidate

Substance Dose (μg/plate) TA97 TA98 TA100 TA1535
- S9 + S9 - S9 + S9 - S9 + S9 - S9 + S9
Py-mulin 1000 46.3 ± 3.5** 55.0 ± 4.6** 21.0 ± 2.6 19.7 ± 3.5 36.7 ± 3.1** 38.0 ± 3.6** 20.7 ± 3.1 27.3 ± 2.5
200 62.0 ± 5.3 62.7 ± 4.2** 22.7 ± 3.1 26.7 ± 2.5 83.7 ± 4.5* 73.3 ± 3.1** 25.0 ± 2.6 38.7 ± 3.5
40 72.0 ± 3.6 77.7 ± 3.2 33.3 ± 4.5 28.0 ± 4.9 93.7 ± 4.2 89.0 ± 8.5 34.3 ± 4.0 41.7 ± 4.7
8 80.3 ± 4.7 79.3 ± 5.1 23.7 ± 4.0 24.7 ± 5.5 105.7 ± 6.7 101.3 ± 3.5 38.7 ± 4.7 36.7 ± 5.0
1.6 76.3 ± 5.6 94.3 ± 4.0 32.7 ± 3.5 31.7 ± 3.1 112.0 ± 5.3 115.0 ± 5.0 42.0 ± 4.4 44.3 ± 5.5
Vehicle control 84.0 ± 5.0 98.3 ± 5.1 24.7 ± 3.5 29.7 ± 2.1 111.3 ± 4.2 120.0 ± 5.6 34.7 ± 4.2 43.3 ± 5.7
Positive control 737.3 ± 13.3** 964.7 ± 13.0** 567.7 ± 11.5** 614.0 ± 15.1** 913.3 ± 20.8** 1212.3 ± 12.9** 583.0 ± 13.7** 746.3 ± 12.6**
  1. Note: The numbers indicate the means and standards deviation values of CFU in triplicate assay systems
  2. + S9: with metabolic activation; − S9: without metabolic activation
  3. Vehicle contro: DMSO (100 μL/plate)
  4. Positive control: 2-aminofluorene for all strains with S9; Fenaminosulf for all strains without S9
  5. *p < 0.05, **p < 0.01 vs. vehicle control