Dexmedetomidine reduces propofol-induced hippocampal neuron injury by modulating the miR-377-5p/Arc pathway

Table 2 Primer sequences of RT-qPCR

Gene	Sequence
DNMT3A	F: AGAAGCCGCTGTTACCTCTT
DNMT3A	R: GCTGAAACCCTTTGCACAGA
Arc	F: CTGACTCACAACTGCCACAC
Arc	R: TGAGGAAGCCAGATCGTGTT
Caspase-3	F: TCACAGCCGCAACTCAGAC
Caspase-3	G: GGCAGGTCCTGATGAGGTG
β-actin	F: GTGACGTTGACATCCGTAAAGA
β-actin	G: GCCGGACTCATCGTACTCC
miR-377-5p	F: ACACTCCAGCTGGGAGAGGTTGCCCTTGGT
miR-377-5p	G: CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGGAATTCAC
U6^a	F: CTCGCTTCGGCAGCACA
U6^a	G: AACGCTTCACGAATTTGCGT
miR-377-5p^b	F: AAAATTTTTTTGGGAGAGTTTTTTC
miR-377-5p^b	G: TTAATAACCATAACCAAACAACGAT
GAPDH^b	F: CCTTCCCACCCTGTTCATCT
GAPDH^b	G: AGTTTAGCTGGCCTGGTGAT

Abbreviations: RT-qPCR reverse transcription-quantitative polymerase chain reaction, F forward primer, R reverse primer
^a U6, coding gene of U6snRNA
^b Primers of promoters

ISSN: 2050-6511