Table 1 Primer sequences for real-time reverse transcription-PCR and plasmid construction
From: Interaction between AhR and HIF-1 signaling pathways mediated by ARNT/HIF-1β
Gene | Sequence |
|---|---|
HIF-1a | |
Forward primer | 5′-TGACTGTGCACCTACTATGTCACTT-3′ |
Reverse primer | 5′-GGTCAGCTGTGGGTAATCCACTC-3′ |
CAIX | |
Forward primer | 5′-GCCGCTACTTCCAATATGAGGG-3′ |
Reverse primer | 5′-AACCAGGGCTAGGATGTCACCA-3′ |
VEGF | |
Forward primer | 5′-GAACTTTCTGCTGTCTTGGGTGCAT-3′ |
Reverse primer | 5′-GGTCTGCATTCACATTTGTTGTGCTG-3’ |
CYP1A1 | |
Forward primer | 5′-ATTGGGCACATGCTGACG-3’ |
Reverse primer | 5′-TGCTGGCTCATCCTTGACAG-3’ |
CYP1B1 | |
Forward primer | 5′-GCCGCTACTTCCAATATGAGGG-3’ |
Reverse primer | 5′-AACCAGGGCTAGGATGTCACCA-3’ |
β-Actin | |
Forward primer | 5′-CACCTTCTACAATGAGCTGCGTGTG-3’ |
Reverse primer | 5′-ATAGCACAGCCTGGATAGCAACGTAC-3’ |
AhR | |
Forward primer | 5′-GAAGATCTATGAACAGCAGCAGCGC-3′ |
Reverse primer | 5′-GGGGTACCCCCAGGAATCCACTGGATGTCAA-3’ |
ARNT | |
Forward primer | 5′- CCGCTCGAGTTCTGGGGAGTGGCCTTTCTT-3′ |
Reverse primer | 5′-CGGGATCCAATTCTGAAAAGGGGGGAAAC-3′ |