Fig. 4

Effect of fasudil on oxidative stress-related markers (SOD, MDA and GSH-Px) in LPS-induced inflammatory injury of rat PMVECs. A, C and E The changes of SOD, MDA and GSH-Px in rat PMVECs after LPS (10 μg/ml) treatment for indicated durations. ^*P < 0.05, ^**P < 0.01 vs the value at 0 h. B, D and F Fasudil reversed the changes of SOD, MDA and GSH-Px induced by LPS in rat PMVECs. Quiescent PMVECs were incubated with vehicle or fasudil for 2 h, followed by stimulation with 10 μg/ml of LPS for 12 h. After the cells were lysated, the activities of SOD and GSH-Px and the content of MDA were measured according to the instructions of manufacturer. Values were presented as means ± SD of four independent experiments. ^##P < 0.01 vs control group; ^*P < 0.05, ^**P < 0.01 vs LPS-alone group