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Fig. 6 | BMC Pharmacology and Toxicology

Fig. 6

From: ROCK inhibitor fasudil reduces the expression of inflammatory factors in LPS-induced rat pulmonary microvascular endothelial cells via ROS/NF-κB pathway

Fig. 6

Effects of N-Ace on the increased mRNA expression of inflammatory factors and the nuclear translocation of NF-κB p65 induced by LPS in rat PMVECs. A N-Ace inhibited the nuclear translocation of NF-kB p65 induced by LPS in rat PMVECs. Quiescent PMVECs were incubated with vehicle or N-Ace for 2 h, followed by stimulation with LPS (10 μg/ml) for 12 h. Values were presented as means ± SD. ##P < 0.01 vs control group; *P < 0.05, **P < 0.01 vs LPS-alone group. B N-Ace inhibited the increased mRNA expressions of IL-6 and MCP-1 induced by LPS in rat PMVECs. Quiescent PMVECs were incubated with vehicle or N-Ace for 2 h before stimulated with LPS (10 μg/ml) for 6 h or 12 h for the detection of the levels of MCP-1 and IL-6 respectively. Intensity of IL-6 and MCP-1 was standardized to that of β-actin. Values were presented as means ± SD of three independent experiments. ##P < 0.01 vs control group; *P < 0.05, **P < 0.01 vs LPS-alone group

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