Fig. 4From: Nalbuphine alleviates inflammation by down-regulating NF-κB in an acute inflammatory visceral pain rat modelThe mRNA and protein levels of IL-1β, TNF-α, and IL-6 in the lumbar spinal cord of rats. a-c The mRNA expressions of IL-1β, TNF-α, and IL-6 in the spinal cord were detected via RT-qPCR. d The protein levels of IL-1β, TNF-α, and IL-6 in the spinal cord were detected via western blotting. GAPDH was used as a loading control. (Data were the mean ± SEM, n = 4). *p < 0.05, AA vs control; #p < 0.05, AA+nalbuphine vs AA, &p < 0.05; AA+PDTC vs AABack to article page