Fig. 3
The effect of EA on FXIIIa-mediated formation of fibrin(ogen) – α2-AP complex. This effect was investigated by western blot assay in which different concentrations of EA (100, 500, 1000, 3000, and 5000 µM) were used. Evidently, EA inhibited the formation of fibrin(ogen) – α2-AP complex at a concentration as low as 100 µM supporting the physiological relevance of the action of EA. In theory, this would mean that the blood clot becomes more susceptible to hydrolysis by the fibrinolytic enzyme plasmin. Original blot is provided in the supplementary information. The edges were removed to provide better clarity. Experiment was repeated three times