Fig. 3

DSF inhibits the activation and viability of primary rHSCs in vitro. A Bright-field images of control and DSF-treated rHSCs at days 3, 6 and 8 of culture. Scale bar, 50 μm. B DSF-treated and control rHSCs were immunostained with α-SMA antibodies and DAPI. Quantified analysis of α-SMA intensities was shown below. *, P < 0.05 Scale bar, 25 μm. C EdU incorporation and quantification. EdU-positive cells were imaged on the left and quantified on the right panel. Scale bar, 50 μm. D Viabilities of rHSCs incubated with different concentrations of DSF **, P < 0.01