Skip to main content
Fig. 4 | BMC Pharmacology and Toxicology

Fig. 4

From: Disulfiram inhibits liver fibrosis in rats by suppressing hepatic stellate cell activation and viability

Fig. 4

DSF inhibits the viability of HSCs but has minimal toxicity on hepatocytes. A Viability of HSC-6 T treated with increasing concentrations of DSF or control. B, C The viability of LX-2 (B) and THLE-2 (C) cells treated with DSF or control. D Cell viabilities of LX-2 only, or LX-2 co-cultured with THLE-2 treated with DSF or vehicle (left), and that of THLE-2 only, or THLE-2 co-cultured with LX-2 treated with DSF or vehicle (right). E, F HSC-T6 cell apoptosis was detected by TUNEL staining. Scale bar, 50 μm. The ratios of apoptotic cells were analyzed below. G Immunofluorescence staining of α-SMA (red) and TUNEL (green) in CCl4-induced fibrotic rat liver tissues. Close-up images were shown in the while rectangle. Scale bar, 25 μm. TUNEL-positive HSCs per field were analyzed on the right. White arrows indicated apoptotic HSCs and white arrowheads indicated hepatocytes. **, P < 0.01

Back to article page