Fig. 2

H & E-stained testicular sections of Busulfan-injected rats: (a) normal spermatogenesis was observed in histological evaluation of testicular tissue sections of the control group with thick-walled seminiferous tubules along with multiple layers of spermatogenesis cells and elongated spermatozoa within or close to the luminal surface. (b) Testicular sections of group II (received 10 mg/kg of Busulfan IP) showed minor evacuation and destruction in the spermatogenesis process with multiple vacuoles, thin-walled seminiferous epithelium; however, spermatozoa were present in the lumen and quite notable numbers of spermatogonial cells in the innermost layer of the seminiferous tubules. (c) Testicular tissue sections of the third group (received 40 mg/kg of Busulfan IP) revealed the normal spermatogenesis process with the presence of spermatogenic cells and elongated spermatozoa seminiferous tubules and no destruction was observed in the tubules. (d) Histological evaluations demonstrated that most of the seminiferous tubules and spermatogenesis cells of testicular tissue are destroyed without any spermatozoa in the lumen and the seminiferous epithelium became thinner with only one layer of germ cells remaining at the periphery of tubules 60 days after a single injection (IT) of 5 mg/kg/rat Busulfan. Images scale bar; Left, 200 μm and right, 50 μm. Abbreviations: L, lumen; Sc, spermatogonia; S, spermatocytes; St, spermatid and Sp, spermatozoa, (n = 8)