Fig. 2

Serum factors in anaphylactic guinea pigs (mean + SD, n = 6). Guinea pigs received 3 doses (10%, 0.5 ml) of intraperitoneal injection for sensitization and a dose (10%, 0.5 ml) of intravenous injection for evocation. On the fourteenth day, the serum was obtained 30 min following evocation. Seven days later (Day 21), their serum samples were also collected. Serum factors, including IgE (A), HIS (B), CRP (C), HMGB1 (D), and C5a (E), were detected using ELISA kits. IgE, immunoglobin E; HIS, histamine; CRP, C-reactive protein; HMGB1, high mobility group protein; and C5a, complement 5a. Com A, Component A (above 100 kDa); Com B, Component B (from 10 to 100 kDa); Com C, Component C (below 10 kDa); Ori, the original TW80; and NS, normal saline for injection. *, P < 0.05 vs NS; #, P < 0.05 vs Ori; ANOVA