Fig. 5
From: Macromolecules in polysorbate 80 for injection: an important cause of anaphylactoid reactions
Substances released from blood leukocytes (mean + SD, n = 3). After guinea pig’s blood leukocytes were prepared, the leukocytes were treated with the original TW80 and its components (16 μg in 5 ml) at 37 °C for 30 min. Factors released from the leukocytes treated for 30 min were measured. β-HEX, β-hexosaminidase; LZM, lysozyme; HIS, histamine. Com A, Component A (above 100 kDa); Com B, Component B (from 10 to 100 kDa); Com C, Component C (below 10 kDa); Ori, the original TW80; and NS, normal saline for injection. C48/80 (20 μg/ml) was served as a positive control. *, P < 0.05 vs NS; # P < 0.05 vs Ori; ANOVA