Fig. 4

Cellular apoptosis after resveratrol (RSV) treatment. A-H, DAPI staining (blue) shows that the nuclear fragments (arrows) is increased in the D-gal group compared with that in the control group, meanwhile, the cell apoptosis is decreased in the RSV groups at D5 and D12. I-P, the caspase-3-positive cells (red) in various groups. Numerous apoptotic cells can be found in embryoid bodys and cerebral organoids of every group at D5 and D12 as well, but the caspase-3-positive cells in the D-gal group are maximum, compared with other groups, RSV can rescue cell apoptosis. Q, statistical analyses show caspase-3-positive cells are increased in the D-gal group compared with that in the other group, and RSV can rescue cell apoptosis significantly in the RSV groups. A-P, scale bars = 20 μm