Fig. 5

Germ layer differentiation after resveratrol (RSV) intervention. A-C, the amounts of Nestin (green) and CXCR4 (red) in vitro at D5, D8 and D12, Nestin-positive cells appear irregular filaments at D5. As the culture time increased, Nestin-positive cells regularly surround the NRs, and CXCR4-positive cells surround into regular circles in the embryoid bodys and cerebral organoids. D-O, the amounts of Nestin and CXCR4 in different groups in vitro at D5 and D12, Nestin and CXCR4 are expressed weakly in the D-gal compared with that in the control and RSV groups (P < 0.05), and RSV can restore the their expression in RSV-L, compared with D-gal group (P < 0.05). P, Q, statistical analyses show, at same age, the expression of Nestin and CXCR4 is weaker and less in the D-gal group compare with that in the control, and RSV can rescue the expression of Nestin and CXCR4 positive cell numbers (P < 0.05). A-C, scale bars = 50 μm; D-O, scale bars = 20 μm