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Table 1 Sequences of primer pairs used in PCR

From: Cytotoxic and apoptotic potential of gemini-chrysophanol nanoparticles against human colorectal cancer HCT-116 cell lines

Gene

Sequence (5′ → 3′)

Amplicon (bp)

Bax

Forward: 5' GCAAACTGGTGCTCAAGG 3'

Reverse: 5' ACTCCCGCCACAAAGA 3'

187

Bcl-2

Forward: 5' TGGGAAGTTTCAAATCAGC 3'

Reverse: 5' GCATTCTTGGACGAGGG 3'

236

P53

Forward:5'CACCTACCTCACAGAGTGCAT 3'

Reverse:5'AAACTACCAACCCACCGACCA 3'

146

β2m

Forward: 5' CTACTCTCTCTTTCTGGCCTG 3'

Reverse: 5' GACAAGTCTGAATGCTCCAC 3

191

  1. bp Base pair
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BMC Pharmacology and Toxicology

ISSN: 2050-6511

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