Table 2 The sequence of primer sets applied for real-time PCR assays
Gene | Primers | TM | Product |
|---|---|---|---|
Heat shock protein 5 (Hspa5/GRP78) | F- CCAGCGACAAGCAACCAAAG R- AGCTGCTGTACTGTGAGGATG | 59.8 | 174 bp |
Activating transcription factor 6 (ATF6α) | F-GATGCCTTGGGAGTCAGACC R-ATGGAGCAACTGGAGGAAGC | 60 | 163 bp |
Eukaryotic translation initiation factor 2 alpha kinase 3 (Eif2ak3/Perk) | F- AGAGATAGATGGGTGGCAAAA R: ATTCGTCCATCTAAAGTGCTG | 56 | 157 bp |
X-box binding protein 1 (XBP1) | F- GGAGCAGCAAGTGGTGGATT R- ATCCAGCGTGTCCATTCCC | 60 | 136bp |
CHOP | F- CTGGAAGCCTGGTATGAGGAT R- CAGGGTCAAGAGTAGTGAAGGT | 59.5 | 185 bp |