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Fig. 1 | BMC Pharmacology and Toxicology

Fig. 1

From: Overexpression of estrogen receptor β inhibits cellular functions of human hepatic stellate cells and promotes the anti-fibrosis effect of calycosin via inhibiting STAT3 phosphorylation

Fig. 1

Efficiency of lentivirus infection and overexpression of ERβ in LX-2 cells. A Lentivirus transfection efficiency of LX-2 cells observed by fluorescence microscope (× 100). To determine the optimal transfection conditions, the ERβ-overexpressed lentivirus with multiplicity of infection (MOI) of 10, 20, 30 and 40 was transfected to LX-2 cells for 48 h (a-d) and 72 h (e-f), respectively. The positive transfected cells were shown in green fluorescence. Stable transfected cells were screened using 2 μg/mL purinomycin for 24 h. B Relative mRNA expression of ERβ detected by quantitative real-time PCR. Intensity of ERβ was standardized to that of β-actin. Data were shown as mean ± SD of 3 independent experiments. C The overexpression of ERβ protein in LX-2 cells was measured by western blot. The β-actin was used for normalization. The experiment was performed in triplicate and repeated three times. # p < 0.05, ###p < 0.001 vs vehicle group

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BMC Pharmacology and Toxicology

ISSN: 2050-6511

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