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Fig. 3 | BMC Pharmacology and Toxicology

Fig. 3

From: Overexpression of estrogen receptor β inhibits cellular functions of human hepatic stellate cells and promotes the anti-fibrosis effect of calycosin via inhibiting STAT3 phosphorylation

Fig. 3

Inhibitory effect of ERβ overexpression and calycosin on the migration of TGF-β1-induced LX-2 cells. A Representative cell fields for each group (× 400). Cell transwell migration assay was applied to cells in control group (a), vehicle group (b), TGF-β1 model group (c), TGF-β1 + calycosin group (d), TGF-β1 + E2 group (e), TGF-β1 + ERβ overexpression group (f), TGF-β1 + ERβ overexpression+ calycosin group (g), and TGF-β1 + ERβ overexpression+E2 group (h). Cells were treated with calycosin (200 μM) or E2 (1μM) for 24 h, and then 10 ng/ml TGF-β1 was added to upper chambers. After 12 h of incubation, all unmigrated cells were removed from the upper surface of the filters and the migrated cells were fixed and stained with crystal violet solution . B The average number of invasive cells per field in each group. Numerical representation of the data was obtained by counting the average number of stained cells from five random fields of each group using a 400-fold microscope. Data are shown as mean ± SD of three independent experiments. ###p < 0.001 vs vehicle group; ***p < 0.001 vs TGF-β1 group; +p < 0.05, ++p < 0.01 vs the corresponding treatment groups without ERβ overexpression

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BMC Pharmacology and Toxicology

ISSN: 2050-6511

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