Fig. 3

Effect of cornin on autophagy post rats CI/R injury through the PI3K/Akt/mTOR signaling pathway. Autophagy-associated proteins were assessed by western blotting using western blot analysis, and the full-length blots/gels are presented in Figure S2. After CI/R, rats were treated with cornin (2.5, 5, 10 mg/kg) for 24 h, and equal amounts of tissue lysate protein (50 μg) were separated by SDS-PAGE and analyzed by western blotting using specific antibodies. LC3-II, p62, Beclin-1, p-mTOR, and p-Akt expression levels were quantified and are represented as the mean ± SD, (n = 3). ^*P < 0.05, ^**P < 0.01 vs. the sham group; ^#P < 0.05, ^##P < 0.01 vs. CI/R group