Fig. 4

Effect of PI3K inhibitor LY294002 on autophagy post rats CI/R injury. The rats were injected with PI3K inhibitor LY294002 (10 mM) before cornin (10 mg/kg) treatment. Autophagy-associated proteins were assessed by western blotting using western blot analysis, and the full-length blots/gels are presented in Figure S3. LC3-II, p62, Beclin-1, p-mTOR, and p-Akt expression levels were quantified and are represented as the mean ± SD, (n = 3). ^*P < 0.05, ^**P < 0.01 vs. the sham group; ^#P < 0.05, ^##P < 0.01 vs. CI/R group; ^ΔP < 0.05, ^ΔΔP < 0.01 vs. cornin 10 mg/kg group