Fig. 5

Protective effects of cornin on rats CI/R inury assessed by immunohistochemistry and western blot. A After 24 h of CI/R injury, the number of cells positive for NeuN, GFAP, LC3-II, and Beclin-1 in the sham group, CI/R group, cornin (10 mg/kg) group, and cornin + LY294002 group was quantitatively evaluated by immunohistochemistry. The magnification was × 200. The data are presented as the mean ± SD, (n = 3). ^**P < 0.01 vs. the sham group; ^##P < 0.01 vs. CI/R group; ^ΔP < 0.05 vs. cornin 10 mg/kg group. B GFAP, Bax and Bcl-2 was assessed by western blotting using western blot analysis, and the full-length blots/gels are presented in Figure S4, and the expression levels were quantified and are represented as the mean ± SD, (n = 3). ^**P < 0.01 vs. the sham group; ^##P < 0.01 vs. CI/R group; ^ΔP < 0.05 vs. cornin 10 mg/kg group