Fig. 2

CXB reduced intracellular ROS and mitochondrial ROS production in NPCs. A Representative images of NPCs treated with IL-1β alone or with 2 µM or 10 µM CXB for 24 h and stained for intracellular ROS and mitochondrial ROS by DCFH-DA and MitoSOX Red at 37˚C, 30 min. B, C CXB reduced intracellular ROS and mitochondrial ROS production at higher concentrations. D, E 10 µM CXB reduced intracellular ROS and mitochondrial ROS production significantly at 24 h. Flow cytometry analysis was calculated as mean fluorescence intensity (MFI). Data were represented as mean with SD. Scale Bars, 200 μm. *P ≤ 0.05, **P ≤ 0.01, *** P ≤ 0.001