Fig. 1

Anti-fibrosis effect of Radotinib (Rb) on TGF-β induced A549 cells. A Chemical structure of Rb. B A549 cells were incubated for 24 h, and then treated with Rb (3–1000 nM) for 24 h. Cytotoxicity was determined using a 2,3-bis[2-methyloxy-4-nitro-5-sulfophenyl]-2 H-tetrazolium-5-carboxanilide assay. C and D The cells were incubated with (+) or without (-) TGF-β1 (10 ng/mL) and Rb (30 nM) for 72 h. The mRNA expression of Snail and Twist were normalized to β-actin. (E-J) Immunocytochemistry assay with E-cadherin (Green color), α-smooth muscle actin (α-SMA, Yellow color) and F-actin (Red color) and DAPI (4′,6-diamidino-2-phenylindole). The bar graphs indicate the intensity of E-cadherin, α-SMA and F-actin, respectively. The untreated group (UN) is expressed as 100%. Data are expressed as means ± standard deviations (n = 3). * p < 0.05 vs. UN; # p < 0.05 vs. TGF-β1