Fig. 2

Radotinib (Rb) inhibits TGF-β1 induced tyrosine kinase signaling pathway in A549 cells. A549 cells were incubated for 24 h, and then stimulated with (+) or without (-) TGF-β1 (10 ng/mL) and Rb (30 nM) for 30 min or 72 h. (A-H) Protein expression was analyzed by western blot and incubated with specific antibodies against phosphorylated (p)-Smad2, Smad2/3, p-ERK, ERK, p-P38, P38, p-AKT, AKT, α-SMA and β-actin. The bar graphs show the intensity of western blot bands. The untreated group (UN) is expressed as 100%. Data are expressed as means ± standard deviations (n = 3). * p < 0.05 vs. UN ; # p < 0.05 vs. only treated with TGF-β1 group