Fig. 2

The effects of GA on MDR-AB infected cell injury. Different concentrations of GA were added into A549 cells, and cytotoxicity was detected by CCK8 assay (A). Various concentrations of MDR-AB were added to A549 cells, and cytotoxicity was measured by LDH (B). The effect of GA on the cell viability of MDR-AB-infected A549 cells was measured by LDH (C). Cell morphology was observed after MDR-AB invasion for 12 h by inverted microscope (D). Apoptosis was detected by flow cytometry (E, F), and the mRNA expression level of Bcl-2 and Bax was detected by RT-PCR (G-I). ^*P < 0.05 vs. MDR-AB group; ^**P < 0.01 vs. MDR-AB group; ^#P < 0.05 vs. control group; ^##P < 0.01 vs. control group