Fig. 4

Effect of GA on MDR-AB infected inflammation of A549 cells. The contents of IL-1β (A), IL-6 (B), and TNF (C) in the supernatant was determined by ELISA after 12 h culture. After 12 h of culture, the supernatant was discarded, and cells were collected. The mRNA expression levels of TNF (D), IL-1β (E), IL-6 (F), TLR-1 (G), TLR-2 (H), TLR-4 (I), TLR-5 (J), TLR-6 (K), TLR-9 (L), IRF3 (M) and MYD88 (N) were detected by RT-PCR. ^*P < 0.05 vs. MDR-AB group; ^**P < 0.01 vs. MDR-AB group: ^#P < 0.05 vs. control group; ^##P < 0.01 vs. control group