Fig. 3

Cisplatin reduced cell viability and affected mitochondrial function in HEI-OC1 cells. A Cell viability was determined using the CCK-8 assay. HEI-OC1 cells were treated with various concentrations (0, 10, 20, 30, 40 µM) of cisplatin for 8, 16, 24, and 48 h. Cell viability decreased with cisplatin treatment in a time-dose-dependent manner. B, C FITC fluorescence intensity was measured using flow cytometry. Cisplatin exposure increased ROS levels. D, E, F Cisplatin treatment impaired mitochondrial function. Data are presented as the mean ± SEM of three independent experiments. *p < 0.05;**p < 0.01;***p < 0.001