Fig. 6

Role of miR-34a/DRP1 in cisplatin-induced ototoxicity in HEI-OC1 cells. Cells were transfected with miR-34a mimic or inhibitor and negative control miRNA and then incubated with 20 µM cisplatin for 24 h. A Cell viability was detected by CCK8 assay. B, C The green fluorescence intensity of ROS detected by flow cytometry. D ATP content was detected by chemiluminescence. E Model summarizing the relationship between miR-34a/DRP1 and mitophagy in cisplatin-induced ototoxicity. Data are presented as the mean ± SEM of three independent experiments. *p < 0.05;**p < 0.01;***p < 0.001;****p < 0.0001