Fig. 6

Effects of lupeol on the immunoglobulin and cytokine expression in AD-induced mice. (A) Blood was obtained from the abdominal aorta of each group of experimental mice after they were sacrificed. The serum levels of total IgE, DFE-specific IgE, and IgG2a were measured using ELISA. (B) Both sides of ears were collected from the experimental mice. RNA was isolated from the ears with an RNAiso Plus kit and synthesized into cDNA. The gene expression was measured by RT-qPCR. (C) Mouse ears were homogenized with a tissue extraction buffer, and the cytokine levels in the supernatants of the ear tissue homogenates were determined by ELISA. Data are presented as the mean ± SEM (n = 5). ^#p < 0.05, ^##p < 0.01, and ^###p < 0.001 versus CON group, *p < 0.05, **p < 0.01, and ***p < 0.001 versus AD-induced group. CON, control; O.D, optical density