Effects of coal-fired PM2.5 on the expression levels of atherosclerosis-related proteins and the phosphorylation level of MAPK in ApoE−/− mice

Background Air pollution increases the morbidity and mortality of cardiovascular disease (CVD). Atherosclerosis (AS) is the pathological basis of most CVD, and the progression of atherosclerosis and the increase of fragile plaque rupture are the mechanism basis of the relationship between atmospheric particulate pollution and CVD. The aim of the present study was to investigate the effects of coal-fired fine particulate matter (PM2.5) on the expression levels of atherosclerosis-related proteins (von Willebrand factor (vWF), Endothelin-1 (ET-1), intercellular adhesion molecule-1 (ICAM-1), and E-selectin, and to explore the role and mechanism of the progression of atherosclerosis induced by coal-fired PM2.5 via the mitogen-activated protein kinase (MAPK) signaling pathways. Methods Different concentrations of PM2.5 were given to apolipoprotein-E knockout (ApoE−/−) mice via intratracheal instillation for 8 weeks. Enzyme-linked immunosorbent assay (ELISA) was used to detect the levels of vWF, ET-1 in serum of mice. Immunohistochemistry was used to observe the expression and distribution of ICAM-1 and E-selectin in the aorta of mice. Western blot was used to investigate the phosphoylation of proteins relevant to MAPK signaling pathways. Results Coal-fired PM2.5 exacerbated atherosclerosis induced by a high-fat diet. Fibrous cap formation, foam cells accumulation, and atherosclerotic lesions were observed in the aortas of PM2.5-treated mice. Coal-fired PM2.5 increased the protein levels of ET-1, ICAM-1, and E-selectin, but there was no significant difference in the vWF levels between the PM2.5-treatment mice and the HFD control mice. Coal-fired PM2.5 promoted the phosphorylation of p38, c-Jun N-terminal kinase (JNK), extracellular signal-regulated kinase (ERK) in aortic tissues of mice. Conclusion Coal-derived PM2.5 exacerbated the formation of atherosclerosis in mice, increased the expression levels of atherosclerosis-related proteins in mice serum, and promoted the phosphorylation of proteins relevant to MAPK signaling pathway. Thus, MAPK signaling pathway may play a role in the atherosclerosis pathogenesis induced by Coal-derived PM2.5.


Background
Air pollution caused 4.1 million deaths globally in 2016 alone and is the sixth highest-ranking risk factor for global mortality [1]. Air pollution increases the morbidity and mortality of cardiovascular disease (CVD) [2][3][4][5]. According to a report of the World Health Organization (WHO), around 17.3 million people die of CVD each year, which accounts for 30% of all deaths [6]. Fine particulate matter (PM 2.5 ) is the main toxic component of atmospheric particulate matter and primarily derives from coal-fired emission and automobile exhaust [7]. Moreover, an estimated 2.9 million deaths was attributed to PM 2.5 in 2013 alone, which is considered as a leading risk factor for global disease [8]. PM 2.5 is one of the primary causes of death in China and accounted for 11.1% of all deaths in China in 2016 [1]. Since the PM 2.5 pollution is mainly caused by coal-burning emission in China [9,10], it is important for future research to further elucidate the effects and mechanisms of coal-fired PM 2.5 on cardiovascular diseases. Atherosclerosis (AS) is a type of CVD [11] and is one of the leading causes of death around the world [12][13][14]. AS is also the pathological basis of most CVD, and the progression of atherosclerosis and the increase of fragile plaque rupture are the mechanism basis of the relationship between atmospheric particulate pollution and CVD [15][16][17]. PM 2.5 increased mortality in individuals with CVD [18] via its contribution to the development of atherosclerosis [19]. Additionally, exposure to PM 2.5 is a fundamental cause of cardiovascular diseases [20]. PM 2.5 has short-term and long-term effect on cardiovascular system [14,21]. Animal studies have suggested that PM 2.5 exposure increases both the area of atherosclerotic plaques and plaque vulnerability in apolipoprotein-E knockout (ApoE −/− ) mice and rats [19,22,23]. Similar results have been found from epidemiological studies, which have revealed that exposure to PM 2.5 increases the development of atherosclerosis in humans [24][25][26]. PM 2.5 presumably exerts atherogenic effects by inducing endothelial damage, mitochondrial injury, inflammatory responses, and oxidative stress [23,27,28]. However, the underlying molecular processes and potential mechanisms remain to be fully elucidated due to the etiological complexity of the atherogenesis [29,30].
Mitogen-activated protein kinase (MAPK) signaling pathways are a series of parallel cascades of serine/ threonine kinase, including extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK), and p38 MAPK [31]. MAPK signaling pathways play a key role in the atherosclerosis development [32], and it deserves further investigation in PM 2.5 -induced atherosclerosis. Thus, the present study aimed at exploring the mechanisms of atherosclerosis induced by coal-fired PM 2.5 and the role of MAPK signaling pathways in this disease progression.

Coal-fired PM 2.5 collection and extraction
Raw coal from a typical coal field (Yinchuan) in China was purchased from state-owned coal mines. The coal samples were broken into pieces and ignited in the stove. PM 2.5 emitted from coal combustion was sampled by the dilution tunnel system, and dilution and sampling continued until the combustion finished [33].
The PM 2.5 filters were extracted with ultra-pure water in an ultrasonic bath. After ultrasonic elution and freeze-drying, coal-fired PM 2.5 suspensions were prepared and stored at − 20°C until they were used for exposure to mice.

Animals and experimental groups
ApoE −/− C57BL/6 J mice represent a common experimental model for atherosclerosis research. Forty ApoE −/− male mice (7-8 weeks old, weight ranged from 18 g to 20 g) were obtained from Beijing Vital River Laboratory Animal Technology Co., Ltd. Mice were housed in a barrier system at a controlled temperature (22 ± 2°C) and a relative humidity 40-70%, with a 12 h:12 h light:dark cycle. All animals were given free access to food and water. Atherosclerosis model groups were fed with a high-fat diet (HFD) consisting of 54% regular chow, 20% sugar, 15% lard oil, 7.8% casein, 1.7% calcium hydrogen phosphate, 1.2% cholesterol, and 0.2% bile salt.
Choosing 6 to 10 mice for each group in general mice experiments to meet the statistical requirements, and we chose the median 8. It not only avoided sample size reduction caused by accidental death during the experiment, but also followed the rules of 3R which contain the reduction of animal usage. After 1 week of acclimatization, mice were divided into the following five groups randomly by using random number table (n = 8) and treated with PM 2.5 or phosphate buffer saline (PBS): (1) normal control group (normal diet + PBS); (2) HFD control group (HFD + PBS); (3) low-dose group (HFD + PM 2.5 0.05 mg/kg of body weight [bw]/week); (4) middle-dose group (HFD + PM 2.5 0.50 mg/kg of bw/ week); and (5) high-dose group (HFD + PM 2.5 5.00 mg/ kg of bw/week). Basing on previously reported study [34] and our laboratory former work, different concentrations of coal-fired PM 2.5 (0, 0.05, 0.50, and 5.00 mg/kg of bw) were given to ApoE −/− mice once a week (at 8.00-11.00 a.m. of Tuesday) via intratracheal instillation. After 8 weeks treatment [21], mice were sacrificed by cervical dislocation under isoflurane anaesthesia. Wholeblood samples kept at room temperature for 30 min after they were collected through aortas, and then they were centrifuged at 3000 g for 10 min. Sera were collected and stored at − 80°C. Aortic root samples were fixed in 4% paraformaldehyde and embedded in paraffin, after which they were used for histopathological and immunohistochemical analyses.

Histopathology
As previously reported [35,36], aortas isolated from all groups were fixed in 4% paraformaldehyde for 48 h and subsequently embedded in paraffin. For histopathological assessment, all samples of aorta root were processed into serial sections with 6-μm thick, and stained with hematoxylin and eosin (H&E).

Immunohistochemistry
Immunohistochemistry was performed as previously described [37]. The paraffin-embedded tissue sections were dewaxed, then immersed in 0.01 mol/L citric acid buffer and heated to boiling in an autoclave for 2 min. After treated with 0.3% hydrogen peroxide solution for 10 min, the tissue sections were blocked with 5% bovine serum albumin (BSA) for 1 h at 37°C. Then they were washed three times with tris buffered saline (TBS), and were then incubated with primary antibodies (rat anti-mouse intercellular adhesion molecule-1 [ICAM-1] [dilution 1:100] and rabbit antimouse E-selectin [dilution 1:25], both purchased from Abcam, UK) for 2 h at 37°C. The tissue sections were then washed three times with TBS, and treated with appropriate horseradish peroxidase (HRP)-conjugated secondary antibodies for 2 h at 37°C. Then they were then rinsed three times with TBS again. Antigenantibody reactions were stained with diaminobenzidine (DAB), and sections were also counterstained with H&E. The expression levels of ICAM-1 and Eselectin were observed with a Nikon E400 microscope under high-power (400×) fields.

Enzyme-linked immunosorbent assays (ELISAs)
The concentrations of Endothelin-1 (ET-1) and von Willebrand factor (vWF) in the mice sera were determined by ELISA kits according to the manufacturer's recommendations (Abcam, UK).

Western blotting
As previously described [38], proteins were extracted with protein lysate, and a BCA protein assay reagent kit (Beyotime Biotechnology, Shanghai, China) was used to detect their concentrations. Proteins were subjected to electrophoresis on sodium dodecyl sulfate (SDS)-polyacrylamide gels and then the target proteins were transferred onto nitrocellulose membranes. Subsequently, the membranes were blocked for 2 h at room temperature with 5% (wt/vol) milk in TBS with 0.05% (wt/vol) Tween-20. Next, The membranes were washed in tris buffered saline with Tween 20 (TBST) for three times and incubated overnight at 4°Cwith specific primary antibodies. Antibodies for p-p38 (4511S), p38 (9212S), p-JNK (4668S), JNK (9252S), p-ERK (9101S), ERK (9102S), and β-Tubulin (2146S) were obtained from Cell Signaling Technology (Danvers, MA, USA). Then the membranes were washed with TBST three times and were incubated for 2 h at room temperature with horseradish peroxidase-conjugated secondary antibody. After being washed in TBST, protein bands were detected with an enhanced chemiluminescence (ECL) detection kit (GE Health, USA), and quantified by densitometry (Tanon-4500).

Statistical analysis
All data were presented as the mean ± standard deviation (SD). Statistical analyses were performed with SPSS 18.0 software. One-way analysis of variance was used to analyze the differences among multiple groups. P value < 0.05 was considered to be statistically significant.

Body weights and organ coefficients
After 8 weeks of PM 2.5 exposure, there was no significant difference in body weights among the experimental groups (Fig. 1). There were also no significant differences in the mediastinal lymph-node weights or coefficients between the normal control group and the HFD control group. However, after 8 weeks of treatment with PM 2.5 , HFD-fed ApoE −/− mice had significantly increased mediastinal lymph-node weights and coefficients compared with those of HFD control mice (Fig. 2). Compared with the measured parameters in HFD control mice, PM 2.5 -treatment did not induce any changes in thymus, spleen, liver, or kidney weights-or in their corresponding organ coefficients-in HFD-fed ApoE −/− mice (not shown).

Histopathology
To examine whether coal-fired PM 2.5 promotes the formation of atherosclerosis in ApoE −/− mice, we exposed them to coal-fired PM 2.5 or PBS for 8 weeks. Crosssections of aortas were stained with H&E (Fig. 3). In the normal control group, the intimal structure was wellorganized and intact. However, the intima was markedly thickened in the HFD control group, and some foam cells were located in the subendothelial layer. Moreover, PM 2.5 treatment exacerbated HFD-induced atherosclerosis. Cross-sections of the PM 2.5 -treated mouse aortas showed atherosclerotic lesions, intimal thickening, fibrous cap formation, and accumulation of foam cells, indicating that coal-fired PM 2.5 promoted the formation of atherosclerosis in mice.

The levels of atherosclerosis-related proteins
Compared with the HFD control group, the vWF levels in blood plasma of PM 2.5 -treatment groups were not increased, but were significantly increased in the normal control group (Fig. 4a). This finding indicated that HFD inhibited the level of vWF in ApoE −/− mice.
The levels of ET-1 in the plasma were significantly increased in PM 2.5 -treatment groups compared with the HFD control group, whereas they were lower in normal control group compared to the HFD control group (Fig.  4b). This finding indicated that PM 2.5 increased the expression of ET-1 in ApoE −/− mice.
Immunohistochemistry revealed a slight increase in the expression of ICAM-1 in the HFD control group relative to that in the normal control group. In addition, PM 2.5 treatment significantly increased ICAM-1 expression compared with that in the HFD group (Fig. 4c).
E-selectin expression was also examined by immunohistochemistry (Fig. 4d). There were no visible Eselectin-positive cells in aortas from normal control mice, while HFD significantly increased E-selectin expression. Treatment with PM 2.5 plus HFD markedly increased E-selectin expression in mouse aortas.

Phosphorylation levels of components of MAPK signaling pathways
To further investigate the mechanism of PM 2.5 -induced atherosclerosis, activation of MAPK signaling pathways was examined. As shown in Fig. 5, the phosphorylation levels of p38 MAPK, ERK1/2, and JNK in PM 2.5 -treatment ApoE −/− mice were significantly increased compared with those in HFD control mice. This finding indicated that coal-fired PM 2.5 increased the phosphorylation levels of p38, ERK1/2, and JNK in mouse aortas. Discussion PM 2.5 presumably exerts atherogenic effects by inducing endothelial damage, mitochondrial injury, inflammatory responses, and oxidative stress [21,23,28,39,40]. In our present study, PM 2.5 -treatment significantly increased lymph-node weights and coefficients compared with those of HFD control group. It indicated that in the process of inflammation induced by coal-fired PM 2.5 immunocytes might be involved in and play role in injury or recovery. So further studies are needed to elucidate potential mechanisms underlying this phenomenon.
Epidemiological and experimental studies have suggested that PM 2.5 exposure is a risk factor which promotes the development of AS [41], and lipids accumulation and fibrous plaques formation in arteries are characters of AS [42,43]. In our present study, cross-sections of PM 2.5 -treated mouse aortas exhibited atherosclerotic lesions, intima thickening, fibrous cap formation, and foam cell accumulation, indicating that coal-fired PM 2.5 promoted the formation of atherosclerosis in mice.
The expression of adhesion molecules on the endothelium increased during the progression of AS, which involve the recruitment of monocytes into the circulation and trans-endothelial migration [44]. Moreover, eficiencies of adhesion molecules would inhibit monocyte migration and AS formation in mice [36]. vWF is a large glycoprotein [45] that can be produced in endothelial cells and megakaryocytes [46][47][48]. vWF involves the adhesion of platelets to endothelial cells [47], which is associated with the formation of thrombus and atherosclerosis [49,50]. But It is still controversial whether vWF plays a key regulatory role in the AS formation induced by coal-fired PM 2.5 . A study showed PM 2.5 could increase vWF in old people, but another stuy showed PM 2.5 could decrease vWF level in rats [51]. In our study, there was no significant difference in the vWF levels between the PM 2.5 -treatment mice and the HFD control mice. Our result was consisted with those in the previous study [52], which showed PM 2.5 could not increase vWF in young adults. Different results may caused by different species and different physical conditions. Therefore, further studies are needed to elucidate whether vWF is really involved in the process of AS formation induced by PM 2.5 or not.
ET-1 is a vasoconstrictor peptide that is synthesized by endothelial cells of the vascular wall [53] and by macrophages [54], and has been demonstrated to be a potent vasoconstrictor [55][56][57][58]. ET-1 directly affects Fig. 2 Effects of coal-fired PM 2.5 on mediastinal lymph nodes in terms of (a) weight, and (b) organ coefficient (compared with HFD control mice, *P < 0.05, **P < 0.01, n = 8) blood vessels and the heart [59] and is implicated in many forms of cardiovascular disease [60][61][62][63]. Studies have demonstrated that the overexpression of ET-1 exacerbates HFD-induced AS in ApoE −/− mice [64,65]. However, it remains unclear how increased ET-1 expression exacerbates atherosclerotic progression in HFD-fed ApoE −/− mice [64]. In the present study, we found that coal-fired PM 2.5 elevated the expression of ET-1 in mice plasma. We can not obtain a very ideal dose-response relationship for this index, there are two reasons may induce such condition. The first is that in the experiment only several mice were used for each group and there must be some sampling error which may influence the representativeness of mean for the population. Second, there is no liner relationship between the dose and the response for this index.
ICAM-1 is a transmembrane glycoprotein [66] and is typically expressed on the surface of endothelial and immune cells [67]. Cell-adhesion molecules, such as ICAM-1, involve in binding and recruitment of circulating leukocytes to the vascular endothelial cells and further migration into subendothelial spaces, which are primary processes of AS [68,69]. Hence, ICAM-1 may play a key role at the initial stage of AS [70][71][72][73]. In the present study, we found that coal- fired PM 2.5 increased the expression of ICAM-1 in ApoE −/− mouse aortas. In addition, a soluble form of ICAM-1 has been found in plasma, which may be involved in the progression of AS [74].
E-selectin is a transmembrane glycoprotein [75] and expressed exclusively on the surface of endothelial cells [76,77]. E-selectin is important for the initial rolling interaction [78][79][80] and subsequent adhesion [81] of leukocytes in the inflamed endothelium, as well as for the transmigration of inflammatory cells to inflammation sites [76], which are critical events in the initiation of AS [82]. Moreover, monocytes are recruited to lipid-rich plaques mediated by E-selectin during the progression of AS [83]. E-selectin is a hallmark of atherogenesis [84][85][86] and is implicated in the destabilization of atherosclerotic plaques [87]. It has been reported that E-selectin is mostly absent in the healthy endothelial cells but is apparently upregulated in aberrant endothelia that are covered with atherosclerotic plaques in mice and humans [86]. Eselectin is associated with PM 2.5 measurements at the day of blood drawing [88]. Our present study found that coal-fired PM 2.5 promoted E-selectin expression, indicating that PM 2.5 may aggravate arteriosclerosis by inducing upregulation of E-selectin. Taken together, the PM 2.5 -induced the changes of multiple proteins expression in the present study suggest that these proteins may link coal-fired PM 2.5 exposure with the formation of atherosclerosis.
MAPK signaling pathways are a series of parallel cascades of serine/threonine kinases [31] that transduce extracellular signals into cells and induce cellular biological responses [89]. MAPK signaling pathways play an important role in regulating the cardiovascular system [90], and they also influence the formation and development of atherosclerosis [91,92]. PM 2.5 increases ET-1 levels and markedly upregulates p-p38 MAPK expression in vascular smooth muscle cells [93]. Studies have shown that cigarettesmoke extracts upregulate the ICAM-1 and E-selectin expressions via phosphorylation of JNK and ERK pathways [94,95]. Moreover, a study demonstrated that PM 2.5 increases the expression of ICAM-1 in human endothelial cells via ERK pathway [96]. In the present study, western blotting showed that coal-fired PM 2.5 induced phosphorylation of p38, JNK, and ERK kinases in mouse aortas. Hence, MAPK signaling pathways may partially link coal-fired PM 2.5 exposure with upregulation of ET-1, ICAM-1 and E-selectin.

Conclusion
Coal-derived PM 2.5 exacerbated the formation of atherosclerosis in mice, increased the expression levels of atherosclerosis-related proteins (ET-1, ICAM-1 and Eselectinin) in mice serum and promoted the phosphorylation of proteins relevant to MAPK signaling pathway. Therefore, We postulate that MAPK signaling pathway may play a role in the atherosclerosis pathogenesis induced by coal-derived PM 2.5 . More researches need to be conducted on the relationship between atherosclerosis-related proteins and MAPK signaling pathway and the underlying mechanism needs to be elucidated further in the future.