Volume 13 Supplement 1

18th Scientific Symposium of the Austrian Pharmacological Society (APHAR)

Open Access

Influence of different Hypericum perforatumL. preparations on pharmacokinetic and pharmacodynamic properties of pentobarbital, diazepam and paracetamol

  • Aleksandar Rašković1Email author,
  • Nebojša Stilinović1 and
  • Momir Mikov1
BMC Pharmacology and Toxicology201213(Suppl 1):A11

DOI: 10.1186/2050-6511-13-S1-A11

Published: 17 September 2012

Background

Herb–drug interactions are an important safety concern and the study was conducted regarding the interaction between the natural top-selling antidepressant remedy Hypericum perforatum and conventional drugs.

Methods

This study examined the influence of acute pretreatment with different H. perforatum extracts on pentobarbital-induced sleeping time impairment of motor coordination caused by diazepam and paracetamol pharmacokinetics in mice. The preparations profile of St. John’s wort was determined using RP-HPLC analysis. Ethanolic extract, aqueous extract, infusion, tablet and capsule of H. perforatum were used in the experiment.

Results

By quantitative HPLC analysis of active principles, it has been proved that H. perforatum ethanolic extract has the largest content of naphtodianthrones: hypericin (57.8 µg/ml) and pseudohypericin (155.4 µg/ml). Pretreatment with ethanolic extract of H. perforatum potentiated the hypnotic effect of pentobarbital and the impairment of motor coordination caused by diazepam to the greatest extent and also increased the paracetamol plasma concentration in comparison to the control group. These results were in correlation to naphtodianthrones concentrations.

Conclusions

The obtained results show a considerable influence of H. perforatum on pentobarbital and diazepam pharmacodynamics and paracetamol pharmacokinetics.

Declarations

Acknowledgements

This work was supported by the Provincial Secretariat for Science and Technological Development, Autonomous Province of Vojvodina, project no. 114-451-2458/2011.

Authors’ Affiliations

(1)
Department of Pharmacology, Toxicology and Clinical Pharmacology, Faculty of Medicine, University of Novi Sad

Copyright

© Rašković et al; licensee BioMed Central Ltd. 2012

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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