Whole adult organism transcriptional profiling of acute metal exposures in male Zebrafish

Hussainzada, Naissan; Lewis, John A; Baer, Christine E; Ippolito, Danielle L; Jackson, David A; Stallings, Jonathan D

doi:10.1186/2050-6511-15-15

BMC Pharmacology and Toxicology

Table 3 Comparison of histopathology, chemistry, toxicity and observed gene response among metals

From: Whole adult organism transcriptional profiling of acute metal exposures in male Zebrafish

Metal	Predicted	Histopathology target	GO biological process	KEGG pathway	Genes	References
All (Ni, Co, Cr)	Effects on heme carriers (respiration)	n/a	Cell cycle regulation and apoptosis	Cell cycle regulation and apoptosis	mcm4, gadd45bl, orc6l	[57]
Ni	Oxidative damage to DNA	None	Oxidative stress response	Oxidative stress response	Periredoxin (zgc:110343), thioredoxin (zgc:92903); txnl1; pdia4; pdia4; pdip5; hif1a; xbp1; abcf2; pfkfb3; il1b; egln3; hk2; hspa5; pdia4, dnajb11, dnaja4, jnajb11, ahsa11, hsp70l, hspe1, hsp90b1, hspa4l	[61–66. 70–91]
			Endoplasmic reticulum (ER) stress and UPR (unfolded protein response)	n/a	dnaja4, jnajb11, ahsa11, hsp70l, hspe1, hsp90b1, hspa4l; hspa5; pdia4; dnajb11	[61–69]
			Protein synthesis and translation (DNA replication processes)	Protein synthesis and translation	mcm4, orc6l; mcm3, mcm5; rbb4	[57–60]
Co	Inducer of hypoxic response	Olfactory organs: inflammatory, degenerative, metaplastic, necrotic lesions	Oxidative stress response	Oxidative stress response	gss (zgc:101574); gclc; zgc:110010; psmd3, psmd7, psma6b, psma5, psmc3, psmc4, psmd11b, psmd1, psmc6, psme3, psmc1b	[40, 72–74]
Co	Inducer of hypoxic response		Inflammation and acute phase stress response	Increased metabolic detoxification	Protein serum amyloid A (zgc:103580 ); il1b; c/ebp; atf3	[75–78]
Cr	Oxidizing agent; induces oxidative stress and reactive oxygen species	Gills: hyperplasia, necrosis, hemorrhage, inflammation	Inflammation and acute phase stress response	Inflammation and acute phase stress response	pcna, nfkbiaa, hamp1, ptgs2a, ptgs2b, ptges; dusp2; dusp5; dusp1; gadd45g; hmox1; socs1; egr2; hpx; il1b; c/ebp; atf3	[52, 53, 75–78]
Cr		Intestine: atrophy of mucosal folds, necrosis, inflammation	Ribosome biogenesis	Cell cycle regulation and apoptosis	ccne2, rrm2, zgc:77806; pcna; stat3, p53	[89–91]
		Pharynx: atrophy, mononuclear cell infiltration	Metabolic depression	Metabolic pathways	g6pca, gys2, fabp1a, abcc2; hnf3 α, hnf3 β, hnf1 β, tcf8, bmal, ppargc1, lxr α, coup-tfi, gata-6	[80–82, 84–88]

Summary of gene responses consistent with histopathology and predicted chemistry and toxicity of metals after 24 hr exposure.

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ISSN: 2050-6511

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