Figure 1

Itk , Txk , and Lck gene expression is modulated by dexamethasone in the T cell hybridoma 3DO. (A) The DNA amplified and isolated by the differential display technique (included in the box) is a 275-bp fragment belonging to intron 5 of the Itk gene in proximity to exon 6. In the original fragment, the first and last 10 bp were identical/complimentary to the OPA (the primer used in differential display) used in the PCR reaction and overlapped only partially with the sequence of intron 5 (data not shown). (B) Itk, Txk, and Lck expression in 3DO cells is modulated by 24-h treatment with dexamethasone (100 nM) as demonstrated by the RNAse protection assay. Fold modulation was calculated by dividing the Itk/β-actin cpm ratio of DEX-treated cells by that of the respective untreated control. Values represent the mean (±SD) of three independent experiments. ***P < 0.001, according to Student’s t-test comparing the levels of kinase mRNA in dexamethasone-treated cells to those in medium-treated control cells.