Low-dose oral cholecalciferol is associated with higher numbers of Helios+ and total Tregs than oral calcitriol in renal allograft recipients: an observational study

Table 2 Pairwise comparisons of p- values of Helios⁺, Helios⁻, CTLA-4⁺, IFNg⁺, and Treg differences, in addition to Treg subset ratios among non-vitamin D, calcitriol and cholecalciferol groups based on Kruskal-Wallis test (n = 139)

	Controls	Non-vitamin D	Calcitriol	Cholecalciferol	p value
No. of subjects	16	69	38	16
Total Tregs*		√	√		0.108
			√	√	0.001
	√		√		<0.001
		√		√	0.016
	√	√			<0.001
	√			√	0.326
Helios⁺ Tregs*		√	√		0.353
			√	√	<0.001
	√		√		<0.001
		√		√	0.001
	√	√			<0.001
	√			√	0.548
Helios⁻ Tregs*		√	√		0.101
			√	√	0.062
	√		√		0.001
		√		√	0.418
	√	√			0.011
	√			√	0.173
CTLA-4⁺ Tregs*		√	√		0.268
			√	√	0.004
	√		√		<0.001
		√		√	0.022
	√	√			<0.001
	√			√	0.207
IFNg⁺ Tregs*		√	√		0.535
			√	√	0.024
	√		√		0.016
		√		√	0.095
	√	√			0.069
	√			√	0.898
Treg/CD4⁺ T cells		√	√		0.872
			√	√	0.002
	√		√		0.004
		√		√	0.002
	√	√			0.003
	√			√	0.861
Treg/CD8⁺ T cells		√	√		0.177
			√	√	0.008
	√		√		0.001
		√		√	0.062
	√	√			0.009
	√			√	0.599
Helios⁺/Helios⁻ Tregs		√	√		0.687
			√	√	0.018
	√		√		0.014
		√		√	0.024
	√	√			0.019
	√			√	0.941

Pairwise comparisons among the four groups. p-values ≤0.008 were considered significant due to Bonferroni correction of α- values for multiple comparisons. Significant p-values are bold printed. Asterisks refer to Tregs with the marker combination of CD4⁺ CD25⁺ CD127⁻ Foxp3⁺

ISSN: 2050-6511