Tube No. | Levels | SQV/PZQ Conc.(μM) | Stock(μl) | DMEM(μl) |
---|---|---|---|---|
1,2 | 1 | 0 | 0 | 100 |
3,4 | 2 | 1.6 | 6.25 | 93.75 |
5,6 | 3 | 3.2 | 12.5 | 87.5 |
7,8 | 4 | 6.4 | 25 | 75 |
9,10 | 5 | 12.8 | 50 | 50 |
11,12 | 6 | 19.2 | 75 | 25 |
13,14 | 7 | 25.6 | 100 | 0 |
 | Total |  | 268.75(μl) | 431.25(μl) |
 | QCs |  |  |  |
15,16 | LQC | 6.4 | 100 | 0 |
17,18 | MQC | 12.8 | 100 | 0 |
19,20 | HQC | 19.2 | 100 | 0 |