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Fig. 1 | BMC Pharmacology and Toxicology

Fig. 1

From: High content imaging quantification of multiple in vitro human neurogenesis events after neurotoxin exposure

Fig. 1

DIV 0 and DIV 14 neural cell morphology and SOX 1 expression quantification. hNP cells were seeded onto 96 well plates at a density of 15,000 cells/well, differentiating hNP cultures were fixed at end of DIV 14 for analysis following immunocytochemistry for HuC/D, SOX 1 and nuclear staining. SOX 1+ cells were then imaged and quantified by Cellomics ArrayScan VTI HCS reader high-content imaging system. a, b: Phase contrast images of neural progenitor (DIV 0) and neuron (DIV 14). Scale bars = 100 μm. c, g: DIV 0 and DIV 14 cells hoechst 33342 staining. d, h: DIV 0 and DIV 14 cells HuC/D staining. e, i: DIV 0 and DIV 14 cells SOX 1 staining. f, j: DIV 0 and DIV 14 cells Pseudo colored images. Arrows indicate invalid cells and excluded from quantification. Scale bars = 50 μm. k: The quantification of SOX 1 in DIV0 and DIV14 differentiation. Values are the means ± SD. *significantly difference between group (P < 0.05)

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BMC Pharmacology and Toxicology

ISSN: 2050-6511

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