Fig. 4

The involvement of Atg16L1 in the negative regulation of RSV on TNF-α induced iNOS mRNA expression in HCAECs. HCAECs were either untreated (CON) or treated by TNF-α 10 ng/ml for 2 h (TNF-α) in the presence or absence of RSV 10 μM (RSV10), control siRNA (siCON) or Atg16L1 siRNA (siAtg16L1). Total RNA was prepared after infection and analyzed by real-time quantitative PCR to estimate amounts of iNOS transcript. The amounts of iNOS mRNA produced and normalized to the corresponding amount of GAPDH transcript were shown as the fold increase over untreated control cells. Results are represented as means ± S.E.M. for at least three determinations from independent experiments. (*p < 0.05 indicates significance, compared to TNF-α stimulation only)