Fig. 1

Sema3A expression in SSC-9 oral cancer line and construction of the LentiSema3A-EGFP vector. a The oral cancer cell line SSC-9 expresses lower Sema3A levels. Western blot was performed to detect protein expression of Sema3A in different cancer cell lines. Lane1, SCC-9; lane 2, R40LN; lane 3, R40P. The experiment was repeated 3 times. b Schematic structure of the backbone and helper plasmids for lentiviral vector construction. c Enzyme digestion of the backbone plasmid. Lane1, 10 Kb marker; lane2, AgeI digestion of the backbone plasmid; lane3, backbone plasmid without digestion. d PCR products of Sema3A. Lane1, 5 kb marker; lane2, PCR products of Sema3A with AgeI site at both ends. e PCR confirmation of successful ligation of backbone plasmid and Sema3A fragments. 1–8 are clones formed by ligation and transformed into E. coli. The results showed that 1–4 and 7,8 clones are correct. f Successful packaging of the Lentisema3A-EGFP vector. a 293 T cells under bright field; b 293 T cells under green fluorescent light. Magnification, ×100