Fig. 2

Effect of Isoliquiritigenin on cell cycle progression of HASMCs. a HASMCs were treated with various concentration of ISL for 48 h, and incorporated with BrdU. BrdU content in cells was detected by enzyme-linked immunosorbent assay. After incubation with or without ISL (10 μM) for 48 h, b HASMCs were stained with PI for cell cycle analysis, c cell extracts were analyzed by Western-blot for detecting expressions of p27, CyclinD1 and CyclinE. Protein levels were calculated as a ratio relative to β-actin and expressed relative to untreated HASMCs. All values represented as mean ± SD, n = 5, *: P < 0.05; **: P < 0.01 compared with untreated HASMCs. #: P < 0.05 compared with 10 μM ISL treated HASMCs