Fig. 3

DEX decreases propofol-induced HT22 cell apoptosis via the miR-377-5p/Arc signaling pathway. A HT22 cell apoptosis detected using Hochest 33,258 staining; B HT22 cell apoptosis rate following exposure to the study drug; C miR-377-5p expression levels quantified using PR-PCR; D HT22 cell viability and proliferation determined using CCK-8 assay; E, protein levels of DNMT3A (F), DNMT3B (G), and Arc (H) measured using western blot analysis, and the full-length blots/gels are presented in Figure S3. I methylation level in the miR-377-5p promoter region detected through MeDIP assay. Each value represents the mean ± SD for n = 3. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. DEX, dexmedetomidine; MeDIP, methylated DNA immunoprecipitation