Fig. 5

Effect of fasudil on the increased protein expression and nuclear translocation of NF-κB p65 induced by LPS in rat PMVECs. A The changes of NF-κB p65 protein expression in the whole cell lysates or nuclear fraction of rat PMVECs after LPS (10 μg/ml) treatment for indicated durations. The β-actin or Histone H₂A were used for normalization respectively. ^**P < 0.01 vs the value at 0 h. B Fasudil inhibited LPS-induced nuclear translocation of NF-κB p65 in rat PMVECs. Quiescent PMVECs were incubated with vehicle or fasudil for 2 h, followed by stimulation with LPS (10 μg/ml) for 12 h. Western blot images were representative of three independent experiments. Values were presented as means ± SD. ^##P < 0.01 vs control group; ^*P < 0.05, ^**P < 0.01 vs LPS-alone group