Fig. 6
From: Macromolecules in polysorbate 80 for injection: an important cause of anaphylactoid reactions
Guinea pig’s back skin was stained with Evans Blue after passive cutaneous anaphylactoid (PCA) reaction (mean + SD, n = 4). A Animal was subcutaneously administered with the original TW80 (Ori) and its components (Com A, B, and C). B Animal was intravenously pretreated with cromolyn sodium (CS) (50 mg/kg), and then treated with the original TW80 (Ori) and its components (10%, 0.15 ml). C Evans Blue in A and B (4 cm2) was determined. Com A, Component A (above 100 kDa); Com B, Component B (from 10 to 100 kDa); Com C, Component C (below 10 kDa); Ori, the original TW80; and NS, normal saline for injection. C48/80 (0.50 mg/ml, 0.15 ml) was served as a positive control. *, P < 0.05 vs NS; # P < 0.05 vs Ori; ANOVA