Fig. 1
From: Resveratrol’s neural protective effects for the injured embryoid body and cerebral organoid
The development of cerebral organoid (COs). A, hiPSCs start to aggregate each other. B, hiPSCs gather together to form embryoid bodies (EB) at 1 hr. after plating. C, EBs cultured at day 5 (D5). D, at day 10 (D10), COs were transferred to Matrigel droplet. E, at day 20 (D20), preliminary CO can be found, and in the periphery of cultivation, some transparent annular buds, presumable NRs, appear (↑). F, at day 32 (D32), the cerebral lamination is formed in COs, the organoid volume increases and the radial epithelium becomes thick, showing the typically laminar structure (↑). A-D, scale bar = 500 μm; E, scale bar = 100 μm; F, scale bar = 50 μm