Fig. 3

Photomicrographs of liver and kidney sections of WIN55, 212–2 treated mice and controls. Control and vehicle groups: a & d, low dose groups: b & e and high dose groups: c& f. Image a shows normal kidney, proximal convoluted tubule (PCT), distal convoluted tubules (DCT) and glomeruli (G). Image b low dose groups showing some degenerative histological changes in the kidney compared to controls. Image c changes are more pronounced in the high dose groups with shrinkage of glomeruli (G). Changes in b &c were in the form of tubular epithelium degeneration and necrosis (N), tubular dilatation (D) with dilatation of urinary space in Bowman’s capsule (arrow) and pyknotic nuclei (p). Image d shows normal hepatocytes with acidophilic cytoplasm (thick arrow) and blood sinusoids in between hepatocytes (thin arrow). Image e reveals vacuolated cytoplasm of hepatocytes (v) in low dose groups. Image f display vacuolated hepatocytes (v) in high dose and some nuclei are pyknotic (arrow). Stain H&E (scale bar = 100 um)