Zinc oxide calcium silicate composite attenuates acute tramadol toxicity in mice

Table 2 Wire hanging time (seconds) of tramadol-intoxicated mice and the effect of different treatments

Treatment	20 mg/kg	40 mg/kg	80 mg/kg	160 mg/kg	320 mg/kg	Tramadol	Diazepam
CS	14.6 ± 4.6*^@	16 ± 4.2*^@	17.14 ± 3.35*^@	20.3 ± 3.9*^@	16.3 ± 3.6	2 ± 0.58	4 ± 3.5
CSFe3	4.3 ± 3.1*	10 ± 3.4*	4.2 ± 0.52*	19.8 ± 4.2^ac*^@	17.3 ± 3.9^ac*^@	2 ± 0.58	4 ± 3.5
CSZn3	11.5 ± 3.4*	8.6 ± 3.5*	23.6 ± 4.1^b*^@	27.3 ± 1.8*^{ab c@}	14.8 ± 3.2*^@	2 ± 0.58	4 ± 3.5

Normal wire hanging time (seconds) was recorded to 28 ± 1.9 s (data not shown). Data are mean ± SEM, n = 7. Significance at p < 0.05 using one-way ANOVA followed by Tukey's multiple range test. ^a For comparison against 20 mg/kg, ^b For comparison against 40 mg/kg, ^c For comparison against 80 mg/kg, ^d For comparison against 160 mg/kg, * For comparison against tramadol, ^@ For comparison against diazepam
CS wollastonite-treated mice, CSFe3 wollastonite doped with 3% iron oxide, CSZn3 wollastonite doped with 30% zinc oxide

ISSN: 2050-6511