Table 1 Primer sequences and qPCR conditions
From: Berberine-loaded albumin nanoparticles reverse aflatoxin B1-induced liver hyperplasia
Gene name | Primer Sequence | Annealing Temperature (°C) | Number of Cycles | Reference |
|---|---|---|---|---|
β -actin | F-5′- CTGACCGAGCTGGCTAC-3′ R-5′-CCTGCTTGCTGATCCACA-3′ | 53.4 | 35 | [20] |
P53 | F-5′- CCTATCCGGTCAGTTGTTGGA-3′ R-5′-TTGCAGAGTGGAGGAAATGG-3′ | 57.3 | 35 | [21] |
PTEN | F-5′- TGAGTTCCCTCAGCCATTGCCT-3′ R-5′-GAGGTTTCCTCTGGTCCTGGTA-3′ | 63.5 | 40 | [22] |
H19 | F-5′- TCAGCTCTGGGATGATGTGGT-3′ R-5′-CTCAGGAATCGGCTCTGGAAG-3′ | 62.5 | 40 | [23] |
IGF-2 | F-5′- ATGTCACCCATGTCACCAAG-3′ R-5′-GGCTTGTGCCAATTAGGTTCT-3′ | 57 | 35 | [24] |