Fig. 1

Relative expressions of YY1 and MDR1 in ADM resistant OS cells. (A) MG-63 and MG-63/ADM cells were treated with different concentrations of ADM ranging from 2 µg/mL to 64 µg/mL, and cell viability was assessed by MTT assay. (B) IC50 value was calculated in MG-63 and MG-63/ADM cells. (C) The mRNA expression levels of YY1 (C) and MDR1 (D) in MG-63 and MG-63/ADM cells were detected via qRT-PCR. (E) YY1 and P-gp protein expressions in MG-63 and MG-63/ADM cells were detected by Western blotting. (F) Quantitative analysis of Western blot and GAPDH as internal reference. All the results were shown as mean ± SD (n = 3), which were three separate experiments performed in triplicate. **P < 0.01, ***P < 0.001